NEBNext FFPE DNA Repair Mix – Repair and Prepare DNA from FFPE Samples

The NEBNext FFPE DNA Repair Mix is specifically designed to repair damaged DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This specialized mix restores nicks, abasic sites, and other DNA lesions, producing high-quality DNA suitable for downstream molecular biology applications such as library preparation for next-generation sequencing (NGS), PCR, and qPCR.

By efficiently correcting common FFPE-associated DNA damage, this kit enhances the reliability and reproducibility of genomic analyses, making it essential for clinical research, oncology studies, and archival sample investigations.

✅ Key Features

• Repairs fragmented and damaged DNA from FFPE samples
• Restores nicks, abasic sites, and oxidative lesions
• Produces high-quality DNA suitable for PCR and NGS library prep
• Compatible with low-input and precious samples
• Optimized for clinical research and archival tissue studies

📦 Kit Contents

• DNA Repair Mix enzymes
• Optimized reaction buffer
• Protocol for FFPE DNA repair

🔬 Applications

• NGS library preparation from FFPE samples
• PCR and qPCR analysis of damaged DNA
• Clinical research and oncology studies
• Archival tissue DNA analysis
• Genomic and functional genomics research

❓ FAQ

1. What type of DNA damage does this kit repair?

👉 It repairs nicks, abasic sites, oxidative damage, and other common lesions in FFPE-extracted DNA.

2. Is it suitable for low-input DNA samples?

👉 Yes, the kit works efficiently with low DNA concentrations, making it ideal for precious or limited samples.

3. Can repaired DNA be used for NGS library preparation?

👉 Absolutely. Repaired DNA is fully compatible with downstream NGS workflows and PCR amplification.