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Bovine 8- Oxo- 2'- deoxyguanosine, ,       8-oxo- Dg ELISA Kit - 96tests

Bovine 8- Oxo- 2'- deoxyguanosine, ,       8-oxo- Dg ELISA Kit - 96tests

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This kit is designed to measure 8-oxo-2'-deoxyguanosine (8-oxo-dG) levels in bovine samples, such as serum, plasma, or other biological fluids. It is a complete 96-well ELISA kit, including pre-coated plates, standards, and necessary reagents for efficient and accurate detection.

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Internal Reference: CELI-66080b
Website URL: /shop/celi-66080b-bovine-8-oxo-2-deoxyguanosine-8-oxo-dg-elisa-kit-96tests-34285

Instructions for Use :

1- Sample Preparation : Dilute serum, plasma, or other sample types as required.

2- Standard Preparation : Prepare a standard curve using the provided standards.

3- Assay Procedure : Add standards and samples to the wells.

4- Incubation : Incubate with the detection antibody as per the protocol.

5- Washing : Wash the wells thoroughly to remove unbound substances.

6- Substrate Addition : Add the substrate solution and incubate until color develops.

7- Reaction Stop : Add stop solution and measure absorbance at the specified wavelength.

8- Data Analysis : Calculate concentrations using the standard curve. 

Note : The measurement of 8-oxo-dG as a biomarker for oxidative DNA damage is supported by research (PubMed ID:12376510). This kit provides a reliable method for accurate quantification in bovine samples.